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Engineered Nuclease

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CRISPR/Cas9

Engineered Nucleases

We believe that the most powerful molecular tools enabling targeted cleavage in genome for efficient genome editing are engineered nucleases. For the last decade, engineered nuclease technologies have been developed exponentially to enable us to edit genome efficiently. There are two essential things in engineered nuclease-mediated genome editing. First thing is targeting specific sequence among billions of DNA bases precisely and second thing is cleavage occurring at targeted sites. Through targeted cleavage, it enables gene knockout, gene correction, gene modification and gene addition at intended location.

CRISPR/Cas9 Technology

Since the discovery of CRISPR/Cas9, the 3rd generation of engineered nuclease, CRISPR/Cas9 has rapidly expanded into various scientific area with extensive increase of interest on its improvement from previous generations and it leads us to much simpler and more precise genome editing methodology. CRISPR/Cas9 is novel, programmable nuclease platform revolutionizing the genome editing technology.
· Key Features
· Structure & Mechanism of CRISPR/Cas9
Adapted from bacterial immune machinery, we developed a CRISPR/Cas9 nuclease platform which consist of a target-specific RNA (guide RNA) and Cas9 protein. In this CRISPR/Cas9 system, single guide RNA plays a key role in finding targeted site where cleavage should be occurred.
· Application
And then Cas9 proteins cut the targeted DNA sites by their nuclease domains. Through targeted cleavage by CRISPR/Cas9 system, it enables gene knockout, gene correction, gene modification and gene addition at intended location. By customizing guide RNA, CRISPR/Cas9 can be programmed to bind and cut a specific site of genome enabling efficient editing of genetic information.


Genome Editing


Gene is a designer of life materials, conductor of life phenomena and developing power of life diversity.

Human Genome Project opened era of reading & understanding of genome in not only human but also all species including animal, plant and microorganism. It has elaborated how to read and translate genome. The key technology is Next Generation Sequencing(NGS) based on Polymerase Chain Reaction(PCR).

The big challenge after Human Genome Project has been done is how to edit genome anyway. So genome editing technology has been developed in terms of activity, specificity and easiness of handle progressively. Now CRISPR/Cas9 system is emerging the ever powerful and exact and easy-to-use tool of genome editing.

CRISPR/Cas9 system can search the specific site of genome and can cleavage the exact target site. The highest advantage of CRISPR/Cas9 system is that we can design and synthesis it easily according to target cleavage site. The potential of genome editing technology is proportional to amount of information on genome we have accumulated. So genome editing technology means that it opens era human can use genome with purpose.

Based on the enormous potential, this technology was nominated as Technology of the year 2011 by Nature Methods, Breakthrough of the year 2013 by Science, Top 10 Innovative Technology of 2014 by MIT Technology Review and Top 10 Emerging Technology by WEF.

The application fo Genome Editing Technology

Research Tools

  • Gene-function study
  • Cell-line/animal model development

Gene-based Therapy

  • Genetic therapeutics for rare diseases
  • platform developent of cell-based therapeutic

Drug discovery

  • Drug candidat screenning
  • Cell-line/animal model development

Molecular Breeding

  • A high value plant/animal for a new trait
  • Novel food source

Useful Proteins

  • Protein drugs
  • Protein for industrial uses

Healthcare

  • Molecular diagnostics
  • Integrative research

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